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Levels of soluble ST2 in serum associated with severity of dengue due to tumour necrosis factor alpha stimulation


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Información de la publicación
Tipo de publicación

Científica

Tipología

Investigación y estudios

Medio de publicación

Impreso: Revista indexada

Resumen

The interleukin-1 receptor-like-1 protein (IL1RL1), also known as ST2, has been shown previously to regulate T-cell function and is produced by T cells and endothelial cells. It was reported recently to be elevated in mild dengue patients during acute disease. The ST2 gene encodes several splice products: L (long), V (short) and s (soluble). A cohort of 38 patients with dengue haemorrhagic fever (DHF) and mild dengue fever (DF) were evaluated using a secreted soluble ST2 (sST2) ELISA. The RNA expression of ST2 was evaluated by real-time quantitative RT-PCR using patients’ peripheral blood mononuclear cells (PBMCs) and in vitro using human umbilical vein endothelial cells (HUVECs) exposed to sera from dengue patients. DHF patients had higher levels of serum sST2, tumour necrosis factor alpha (TNF-a), interleukin (IL)-8 and IL-10  compared with DF patients and normal healthy control individuals. However, viraemia was indistinguishable between mild and severe cases. No changes in ST2 mRNA expression were found in PBMCs from these two groups of dengue patients. In vitro, sST2 was elevated in HUVECs treated with patient sera. Neutralization of TNF-a in patient sera by pre-treatment with a TNF-a antibody inhibited the upregulation of sST2 expression in HUVECs. These results implicate serum TNF-a in the modulation of expression of sST2 in an in vitro system, and indicate that sST2 could be associated with the severity of disease. Further studies to determine whether sST2 levels are predictive of the severe form of the disease and the role of sST2 in immune regulation are warranted.

Autores

Natalia Houghton-Triviño, Doris M. Salgado, Jairo A. Rodríguez, Irene Bosch, Jaime E. Castellanos

Registro ISSN

0022-1317

SNIES Área

Immunology and Microbiology

SNIES Categoría

Microbiology

Fecha de publicación 01 de marzo de 2010
Fecha de aceptación 31 de octubre de 2009
Medio indexado (nombre)

Pubmed

Bases de datos donde está referenciada

Pubmed

Información de apoyo a la difusión
Documentos JGV_Houghton-Trivino_N_et_al__2010_1_.pdf

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