Transcription of gingipains in a model of endothelial cells adhesion. Corto (resumen)
Información de la publicación
|Información de la publicación|
|Tipo de publicación||
Investigación y estudios
|Medio de publicación||
Impreso: Revista indexada
Background: Periodontitis has been associated to a risk increased for cardiovascular disease. Transitory bacteremia is an important source of microorganisms in the vascular compartment. Gingipains are proteases produced by P. gingivalis which are very important for its metabolism and function as collaborating molecules in bacterial adhesion to epithelial cells. The roll in the endothelial adhesion is not clear.
Objective: The aim of this study was to evaluate in a model of endothelial cells adhesion the genetic expression of P. gingivalis gingipains rgpA, rgpB y kgp from peripheral blood and subgingival plaque bacteria isolates from periodontitis patients.
Materials and methods: Cells EAhy-926 monolayers were incubated with each one of P. gingivalis isolates (2 reference strains and 14 clinical isolates) in order to evaluate the adhesion process after 30 minutes (using colony forming units count) and the transcription changes for each one of gingipains genes, using real time PCR.
Results: rgpA, rgpB y kgp gene expression of the reference strains showed negative regulation when they were incubated in 5% CO2 if compared with anaerobic incubation. Two of the clinical isolates showed different adhesion percentage depending of its origin (blood or plaque). In general, the adhesion capacity was higher in blood isolates. Contact with endothelial cells induced negative regulation in rgpA, rgpB y kgp in both, bacteria from patients and reference strains when each was compared with bacteria that were not incubated in co-culture with EAhy-926 cells. Conclusions: Contact of bacteria with endothelial cells reduces the gingipains gene expression. This suggest that although the adhesion domains in this proteins could participate in cell adhesion, in this model gingipains do not seem to be involved in this process.
DIANA MARCELA CASTILLO PERDOMO
|Fecha de publicación||17 de julio de 2010|
|Fecha de aceptación||01 de diciembre de 2009|
|Medio indexado (nombre)||
Journal of dental research.
|Bases de datos donde está referenciada||
|Información de apoyo a la difusión|
88th General Session and exhibition of th...ona, Spain
International association for dental research
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