Serum inflammation markers in periodontitis after scaling and root planing. Corto (Resumen)
Información de la publicación
|Información de la publicación|
|Tipo de publicación||
Investigación y estudios
|Medio de publicación||
Impreso: Revista indexada
Objectives: Periodontitis patients show elevated serum levels of inflammatory markers which undergo a significant increase after periodontal treatment, possibly associated with bacteremia induced by anaerobic microorganisms. The purpose of this study was to evaluate the induction of pro-inflammatory cytokines, C-reactive protein (CRP) and Toll-like Receptors (TLRs) in monocytes from patients with periodontitis after an episode of scaling and root planning.
Methods: The participants of this study were eighteen patients diagnosed with generalized chronic periodontitis or generalized aggressive periodontitis showing periodontopathic bacteria in blood induced by periodontal treatment. Peripheral blood samples were taken before and after 8 hours of scaling and root planning, monocytes were isolated, HLA-DR receptor was identified, and TLR-2, TLR-4 and intracellular staining was performed to identify pro-inflammatory cytokines expression by flow cytometry. CRP levels were measured by chemiluminescence assay. Data obtained were analyzed by Wilcoxon test.
Results: Cytokine and surface marker levels showed statistical differences for TNFα and IL1β on CD14/HLA-DR+ monocytes. CRP levels were elevated too (p<0.05). This ex vivo model makes evident a basal stimulus due to passage of anaerobic bacteria to peripheral blood in periodontitis patients, maintaining increased levels of cytokines and CRP. This may reflect the ability of each subject to provide a natural confront against bacterial challenges associated with periodontitis, as expression of a systemic response. Although IL6 and TLRs expression was not significant, it showed an increase after eight hours.
Conclusions: The results support a general tendency to increased cytokines levels in activated monocytes after periodontal treatment. TLRs and HLADR expression reflects a previous stimulus which is maintained across the time and might indicate that bacteremia can be an important factor of cytokines expression. Other factors such as surgical trauma could be associated with this response.
C. ROMERO-SANCHEZ, J. DE AVILA QUIROGA, M. TORRES, A. GONZÁLEZ, and G.I. LAFAURIE
|Fecha de publicación||17 de junio de 2010|
|Fecha de aceptación||01 de noviembre de 2009|
|Medio indexado (nombre)||
Journal of dental research.
|Bases de datos donde está referenciada||
|Información de apoyo a la difusión|
88th General Session and exhibition of th...ona, Spain
International association for dental research
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