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In-vitro Mesenchymal Stem Cells Phenotype On Human dental pulp. Corto (resumen)


Información de la publicación

Información de la publicación
Tipo de publicación

Científica

Tipología

Investigación y estudios

Medio de publicación

Impreso: Revista indexada

Resumen

Tissue engineering and biomimetic approaches are geared toward tooth regeneration after injuries or traumatisms. Due to the high prevalence of dental pulp and periapical tissue diseases causing irreversible damage is necessary to propose new therapeutic approaches to achieve tooth regeneration. The generation of dental structures and/or entire teeth in the laboratory depends upon the isolation and manipulation of stem cells. It has been suggested the existence of postnatal human dental pulp stem cells; important in the mechanisms of tooth regeneration. Dental pulp stem cells can be identified and isolated from a mixed population of cells using techniques as flow cytometry to detect CD73+ and CD105+ markers and the absence of CD34, CD45, in healthy human dental pulp. Objective: To evaluate in vitro expression of CD/73+ / CD105+ / CD34-/CD45- in samples of healthy human dental pulp. Methods: We conducted 9 primary cultures from human dental pulp samples from healthy adult patients and evaluated this phenotype by flow cytometry technique. Results: The primary cultures consisted of dental pulp cells with fibroblast-like morphology. They revealed the presence of the CD73+, CD105+ low markers and hematopoietic stem cells markers and adult lymphocyte CD34+ and CD45-low. Conclusions: The mesenchymal stem cells in the pulpodentinal complex comprise various subpopulations at different stages of differentiation. The development of biological approaches for dental regeneration using populations of dental pulp stem cells is promising.

Autores

JUAN CARLOS MUNEVAR-NIÑO
JORGE FORERO
GLORIA INES LAFAURIE

Registro ISSN

1544-0591

SNIES Área

Dentistry

SNIES Categoría

Dentistry (miscellaneous)

Fecha de publicación 01 de abril de 2009
Fecha de aceptación 04 de enero de 2009
Medio indexado (nombre)

Journal of dental research.

Bases de datos donde está referenciada

PubMed

Información de apoyo a la difusión
Enlaces 87th General Session and exhibition of th...i, Florida
International association for dental research

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