Detection of Porphyromonas gingivalis in bacteremia using different microbiological diagnostics. Corto (resumen)
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|Tipo de publicación||
Investigación y estudios
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Impreso: Revista indexada
Objectives: Molecular microbial diagnosis for detecting Porphyromonas gingivalis during bacteremia after scaling and root planning (SRP) has been not previously reported. The aim of this study was to evaluate Porphyromonas gingivalis in peripheral blood before and after SRP using two microbiological diagnostic approaches: polymerase chain reaction (PCR) and culture.
Methods: 42 patients with severe generalized chronic and aggressive periodontitis being positive for Porphyromonas gingivalis in subgingival plaque were included. In each subject, 4 samples of peripheral blood were drawn from the cubital vein at different times: Pre-treatment: immediately before the SRP procedure (T1), immediately after (T2), 15 minutes (T3) and 30 minutes post-SRP (T4). The samples were taken in duplicate and were analyzed using anaerobic cultures and PCR for the detection of periodontal pathogens. Anaerobic hemo-culture and biochemical and enzymatic tests were applied in order to identify the presence of Porphyromonas gingivalis. For PCR technique the modified phenol-chloroform method and nested-PCR were used to extract and amplify DNA respectively using RNA 16s gene universal bacterial primers, which were then re-amplified with Porphyromonas gingivalis specific primers.
Results: Porphyromonas gingivalis was detected in 48% (20/42) of patients in peripheral blood either in culture, PCR or both techniques. 12% (5/42) patients showed Porphyromonas gingivalis before SRP only by PCR. This pathogen was detected in blood by both techniques after SRP in 45.2% (19/42) of the subjects. Positive results were found in 21.4% (36/168) of blood samples analyzed at all different times. PCR detected the microorganism in 28 blood samples while culture identified it only in 16 samples. There were, however, positive blood samples by culture that were negative by PCR.
Conclusions: PCR was more sensitive in detecting Porphyromonas gingivalis in blood samples than culture during bacteremia.
D.M. CASTILLO, M. SÁNCHEZ-BELTRÁN, J.E. CASTELLANOS, R. DE LEÓN, I. MAYORGA-FAYAD, M. SANZ, and G.I. LAFAURIE
|Fecha de publicación||04 de abril de 2009|
|Fecha de aceptación||04 de enero de 2009|
|Medio indexado (nombre)||
Journal of dental research.
|Bases de datos donde está referenciada||
|Información de apoyo a la difusión|
87th General Session and exhibition of th...i, Florida
International association for dental research
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