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Enterobacteriaceae In Mouth And Exogenus Sources Of Contamination


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Científica

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Investigación y estudios

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Impreso: Artículo corto T4

Resumen

Objectives: Enterobacteriaceae are normal flora of the gastrointestinal tract of humans, are transiently flora in mouth and are important pathogens of the human body. The main factors for oral contamination by enterobacterias in mouth are poor hygiene, fecal-oral contamination, self-inoculation with toothbrushes and the use of antibiotics. The purpose of this study was to evaluate the risk factors for colonization of Enterobacteriaceae in saliva including exogenous sources of contamination and the clonal relationship between species isolated from saliva with those isolated from hands and toothbrushes.

Methods: 150 adult patients were clinically evaluated to identify the presence of Enterobacteriaceae in saliva and hands at two different times and their presence in toothbrushes. The risk factors associated with this contamination were evaluated: sociodemographic factors, systemic diseases, medications, periodontal status, smoking and habits of oral hygiene, toothbrush replacement and hand washing. Subjects with antibiotic intake within 45 days before this study were excluded. Pulsed-field gel electrophoresis was performed to evaluate the clonal relationship between species isolated from saliva, tooth brushes and hands. Bivariate analysis was done with the Chi square test and logistic regression analysis (p<0.05).

<!--0--> Results: 61.3% of subjects had enteric rods in saliva, with Enterobacter cloacae and Klebsiella pneumoniae being the most frequent ones. A significant association was observed between enteric rods in saliva with enterobacterial contamination of toothbrushes (OR:3.4 IC95 1.6 -7) and male gender (OR:2.3 IC95 1.09-4.8). Removal of the toothbrush from the bathroom was a protective factor (OR:0.42 IC95 0.2-0.8). Klebsiella pneumoniae showed the highest clonal relationship among isolates of saliva and toothbrushes. Clonal relationship was observed among isolates of Klebsiella pneumoniae and Enterobacter cloacae isolated from saliva in two different times.

Conclusions: Toothbrushes are the main source of contamination of Enterobacteriaceae in the mouth. The enteric bacteria remain stable in saliva. Keeping toothbrushes outside the bathroom protects from contamination.

Autores

G. LAFAURIE
L.V. MILLÁN OSPINA
M.A. SABOGAL BASSIL
D.M. CASTILLO PERDOMO
J.F. DURAN ANTOLINES,

Registro ISSN

1544-0591

SNIES Área

Dentistry

SNIES Categoría

Dentistry (miscellaneous)

Fecha de publicación 11 de mayo de 2012
Fecha de aceptación 02 de marzo de 2012
Medio indexado (nombre)

Journal of dental research.

English information
Title

Enterobacteriaceae In Mouth And Exogenus Sources Of Contamination

Abstract

Objectives: Enterobacteriaceae are normal flora of the gastrointestinal tract of humans, are transiently flora in mouth and are important pathogens of the human body. The main factors for oral contamination by enterobacterias in mouth are poor hygiene, fecal-oral contamination, self-inoculation with toothbrushes and the use of antibiotics. The purpose of this study was to evaluate the risk factors for colonization of Enterobacteriaceae in saliva including exogenous sources of contamination and the clonal relationship between species isolated from saliva with those isolated from hands and toothbrushes.
Methods: 150 adult patients were clinically evaluated to identify the presence of Enterobacteriaceae in saliva and hands at two different times and their presence in toothbrushes. The risk factors associated with this contamination were evaluated: sociodemographic factors, systemic diseases, medications, periodontal status, smoking and habits of oral hygiene, toothbrush replacement and hand washing. Subjects with antibiotic intake within 45 days before this study were excluded. Pulsed-field gel electrophoresis was performed to evaluate the clonal relationship between species isolated from saliva, tooth brushes and hands. Bivariate analysis was done with the Chi square test and logistic regression analysis (p<0.05).
Results: 61.3% of subjects had enteric rods in saliva, with Enterobacter cloacae and Klebsiella pneumoniae being the most frequent ones. A significant association was observed between enteric rods in saliva with enterobacterial contamination of toothbrushes (OR:3.4 IC95 1.6 -7) and male gender (OR:2.3 IC95 1.09-4.8). Removal of the toothbrush from the bathroom was a protective factor (OR:0.42 IC95 0.2-0.8). Klebsiella pneumoniae showed the highest clonal relationship among isolates of saliva and toothbrushes. Clonal relationship was observed among isolates of Klebsiella pneumoniae and Enterobacter cloacae isolated from saliva in two different times.
Conclusions: Toothbrushes are the main source of contamination of Enterobacteriaceae in the mouth. The enteric bacteria remain stable in saliva. Keeping toothbrushes outside the bathroom protects from contamination.

Keywords

Klebsiella pneumoniae, Microbiology, Saliva and Toothbrushes

Información de apoyo a la difusión
Enlaces IADR 90th General Session. 2012
Link a la publicacion de abstract IADR. 2012

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