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Characterizacion of macrolide resistance in pneumococci, staphylococci and enterococci from Colombian hospitals: A country servillance


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Tipo de publicación

Científica

Tipología

Investigación y estudios

Medio de publicación

Impreso: Artículos de investigación científica o tecnológica T1

Resumen

Objective: The characterization of macrolide resistance in Gram-positive cocci recovered from Colombian hospitals.

Methods: The resistance profiles and mechanism of macrolide resistance were investigated in isolates of Streptococcus pneumoniae (1679), Staphylococcus aureus (348), coagulase-negative staphylococci (CoNS) (175), and Enterococcus spp (123). Minimum inhibitory concentrations (MICs) for erythromycin (ERY) and clindamycin (CLI), detection of macrolide resistance genes, phenotypic characterization, and pulsed field gel electrophoresis (PFGE) of macrolide-resistant pneumococci were performed.

Results: Resistance to ERY and CLI was 3.3% and 2.3% for S. pneumoniae, 58% and 57% for S. aureus (94% for both compounds in methicillin-resistant Staphylococcus aureus (MRSA)), and 78.6% and 60.7% in methicillin-resistant Staphylococcus epidermidis, respectively. ERY resistance was 62% in Enterococcus faecalis and 82% in Enterococcus faecium. The MLSB-type accounted for 71% of S. pneumoniae and 100% of MRSA. The erm(A) gene was prevalent in MRSA, erm(B) in S. pneumoniae and enterococci, and erm(C) in CoNS isolates. Efflux pump genes (mef(A) genes) were mostly identified in S. pneumoniae (24%). The most common genotype amongst ERYresistant pneumococci was the Spain6B-2 clone.

Conclusions: The prevalence of macrolide resistance is low in Colombian pneumococci and high in MRSA (cMLSB-type).

Autores

Jinnethe Reyes, Marylin Hidalgo, Lorena Diaz, Sandra Rincon, Jaime Moreno, Natasha Vanegas, Elizabeth Castañeda, Cesar A. Arias

Registro ISSN

12019712

SNIES Área

Medicine

SNIES Categoría

Microbiology (medical)

Fecha de publicación 20 de febrero de 2015
Fecha de aceptación 20 de febrero de 2015
English information
Title

Characterizacion of macrolide resistance in pneumococci, staphylococci and enterococci from Colombian hospitals: A country servillance

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