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Design of two molecular methodologies for the rapid identification of Colombian Community-Acquired Methicillin-Resistant Staphylococcus aureus (CA-MRSA) isolates


Información de la publicación

Información de la publicación
Tipo de publicación

Científica

Tipología

Investigación y estudios

Medio de publicación

Impreso: Artículos de investigación científica o tecnológica T1

Resumen

Introduction. Community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) isolates are increasing the frequency of infections in healthy persons in the community and hospitalized patients. In Colombia and the Andean region, CA-MRSA isolates have a genetic background that is related to the pandemic USA300 clone.Objective. To design and standardize two molecular methodologies for the rapid differentiation of Colombian community acquired and hospital acquired methicillin-resistant Staphylococcus aureus (HA-MRSA) isolates. Materials and methods. Two molecular methodologies were standardized for the identification of CA-MRSA isolates. The first methodology is based in the differential digestion of the carbamate kinase (arcC) and guanylate kinase (gmk) genes in the sequences type 5 (ST5) and 8 (ST8), present in the HAMRSA and CA-MRSA isolates respectively. The second methodology is based on the PCR amplification of the 5 specific virulence factors found in CA-MRSA and HA-MRSA isolates. The specificity and exactitude of each methodology were evaluated using 237 clinical MRSA isolates. Results. The first methodology identified 100% and 93.2% of the CA-MRSA and HA-MRSA isolates, respectively. The second methodology correctly identified the two isolates types (CA-MRSA and HA-MRSA). Conclusions. These two methodologies are a convenient alternative for the rapid identification of the CA-MRSA isolates in Colombia, compared with other techniques such as pulsed field gel electrophoresis and multilocus sequence typing, which take longer and are more expensive.

Autores

Escobar P. J., Murillo N. M., Gómez M. I., Castro C. B., Chavarro P. B., Márquez O. A., Vanegas N.

Registro ISSN

01204157

SNIES Área

Medicine

SNIES Categoría

Microbiology (medical)

Fecha de publicación 20 de febrero de 2015
Fecha de aceptación 20 de febrero de 2015
Bases de datos donde está referenciada

Scopus, scielo, lilacs, Redalycs

English information
Title

Design of two molecular methodologies for the rapid identification of Colombian Community-Acquired Methicillin-Resistant Staphylococcus aureus (CA-MRSA) isolates

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