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Emerging and existing mechanisms co-operate in generating diverse b-lactam resistance phenotypes in geographically dispersed and genetically disparate Pseudomonas aeruginosa strains


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Tipo de publicación

Científica

Tipología

Investigación y estudios

Medio de publicación

Impreso: Artículos de investigación científica o tecnológica T1

Resumen

 Abstract: β-lactam resistance is driven by a number of mechanisms in Pseudomonas aeruginosa clinical isolates. While several are understood, how they act cooperatively in pathogenic strains is less clear. In some isolates, resistance profiles cannot always be explained by identifying the common resistance determining pathways, suggesting that other mechanisms may be important. Pathogenic P. aeruginosa isolates from four countries were characterized by PCR. Also, quantitative expression analysis for activity of several pathways that influence antibiotic resistance was assessed and culture experiments were conducted to test how random transposition of the insertion sequence IS26 during growth may influence resistance to some antibiotics. In most strains antibiotic resistance was being driven by changes in multiple pathways and by the presence or absence of genes acquired by Lateral Gene Transfer. Multiple mechanisms to resistance were prevalent in strains from all the countries examined although regional differences in the type of interacting mechanisms were apparent. Changes in chromosomal pathways included overexpression of AmpC and two efflux pumps. Also, gain or loss of IS26 at some chromosomal locations, most notably oprD, could influence resistance to carbapenems. IS26 related resistance was found in strains from Argentina and geographically linked Uruguay but not in strains from either Colombia or Australia. P. aeruginosa pathogenic strains are evolving to become multi drug resistant in more complex ways. This is being influenced by single strains acquiring changes in numerous known pathways as well as by newly emerging resistance mechanisms in this species.

Autores

Elena Martinez, Javier Escobar Perez, Carolina Marquez, Elisabet Vilacoba, Daniela Centron, Aura L. Leal, Carlos Saavedra, Sandra Y. Saavedra, Catalina Tovar, Natasha Vanegas, H.W. Stokes.

Registro ISSN

22137165

Fecha de publicación 20 de febrero de 2015
Fecha de aceptación 20 de febrero de 2015
Medio indexado (nombre)

Elsevier

English information
Title

Emerging and existing mechanisms co-operate in generating diverse b-lactam resistance phenotypes in geographically dispersed and genetically disparate Pseudomonas aeruginosa strains

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Enlaces Emerging and existing mechanisms co-opera...sa strains

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