Inicio


Publicación

Molecular epidemiology and characterization of virulence genes of community-acquired and hospital-acquired methicillin-resistant Staphylococcus aureus isolates in Colombia


Información de la publicación

Información de la publicación
Tipo de publicación

Científica

Tipología

Investigación y estudios

Medio de publicación

Impreso: Artículos de investigación científica o tecnológica T1

Resumen

 Objective: To determine the molecular epidemiology and presence of virulence genes in community-acquired and hospital-acquired methicillin-resistant Staphylococcus aureus isolates and their relation to clinical outcomes.

Methods: An observational and prospective study of infections caused by MRSA was conducted between June 2006 and December 2008 across seven hospitals in three Colombian cities. MRSA isolates were analyzed by SCCmec, pulsed-field gel electrophoresis and multilocus sequence typing, and 25 virulence genes were identified. Results: 270 isolates were collected from 262 adult hospital patients with MRSA infection. Overall, 68% were classified as HA-MRSA and 32% as CA-MRSA. We identified differences in the patterns of virulence genes: 85% of HA-MRSA isolates possess the enterotoxin gene cluster (egc), whereas 92% of CA-MRSA isolates possessed the luKF-PV/lukS-PV genes. Multi-varied analysis showed an increased risk of mortality by seg (p=0.001, OR 4.73) and a protective effect for eta (p=0.018, OR 0.33).

Conclusions: Our study confirms that three clones of MRSA predominantly circulate in Colombia: a Chilean clone, a pediatric clone that causes HA-MRSA infections and a USA300-related clone (SCCmec IVc) in CA-MRSA infections, which differ in the expression of clinically important virulence genes. This study confirms that PVL is not a determinant of severity or mortality in CA-MRSA infections.

Autores

Bibiana Chavarro Portillo, Jaime Enrique Moreno, Nancy Yomayusa, Carlos Arturo Alvarez, Betsy Esperanza Castro Cardozo, Javier Antonio Escobar Perez, Paula Lucia Dıaz, Milciades Ibañez, Sebastian Mendez-Alvarez, Aura Lucia Leal,
Natasha Vanegas Gomez.

Registro ISSN

12019712

SNIES Área

Medicine

SNIES Categoría

Infectious Diseases

Fecha de publicación 20 de febrero de 2015
Fecha de aceptación 20 de febrero de 2015
Bases de datos donde está referenciada

Scopus

Información de apoyo a la difusión
Enlaces http://www.ijidonline.com/article/S1201-9...7/abstract

Información de contacto

Contacto de Publicaciones