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QUANTIFICATION OF TWO ISOMERIC FLAVONES IN RAT COLON TISSUE USING REVERSE PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY


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BMC Research Notes, Whitted et al. BMC Res Notes (2017) 10:29, DOI 10.1186/s13104-016-2358-y QUANTIFICATION OF TWO ISOMERIC FLAVONES IN RAT COLON TISSUE USING REVERSE PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY Crystal L. Whitted1, Victoria E. Palau1, Ruben D. Torrenegra2, Oscar E. Rodriguez3 and Sam Harirforoosh1* Abstract Background: Antineoplastic activity has been previously shown for two isomeric flavones, 5,7-dihydroxy-3,6,8- trimethoxy flavone (flavone A) and 3,5-dihydroxy-6,7,8-trimethoxy flavone (flavone B), against colon cancer cell lines ( Thomas et al. in PLoS ONE 7:e39806, 5). Here, we present modified methods for the extraction and quantification of flavones A and B in rat colon tissue after intravenous dosing via high performance liquid chromatography, from the originally described procedure for extraction and quantification in rat plasma ( Whitted et al. in J Chromatogr B Analyt Technol Biomed Life Sci 1001:150–155, 7). Results: Modifications included tissue homogenization (1 g tissue: 2 mL water), filtration of the supernatant with a PVDF membrane, and the use of only one calibration curve to determine the concentration of each flavone in colon tissue. Good separation was achieved and representative equations were linear with r2 ≥ 0.99 for both flavones. Preci- sion and accuracy for flavone A ranged from 0.88–24.03 and 109–116%. Precision and accuracy for flavone B ranged from 1.62–33.56 and 98–113%. Concentrations of 1639 ± 601 ng/g flavone A and 5975 ± 2480 ng/g of flavone B were detected in rat colon tissue 6 h post dosing. Conclusions: Modifications to the extraction methods for flavone A and flavone B from rat colon tissue had good separation, precision, and accuracy. Keywords: 5,7-Dihydroxy-3,6,8-trimethoxy flavone, 3,5-Dihydroxy-6,7,8-trimethoxy flavone, HPLC, Colon, Flavonoids, Cancer

Autores

Crystal L. Whitted1 , Victoria E. Palau1 , Ruben D. Torrenegra2 , Oscar E. Rodriguez3 and Sam Harirforoosh1*

1Department of Pharmaceutical Sciences, Gatton College of Pharmacy Department of Internal Medicine, Quillen College of Medicine, East Tennessee State University, Johnson City, TN 37614. 2Faculty of Science and , Universidad de Ciencias Aplicadas y Ambientales, Bogotá, Colombia 3Department of Environmental Engineering, Faculty of Engineering, Universidad El Bosque, Bogotá, Colombia.

DOI Artículo Digital BMC Research Notes
BMC Research Notes, Whitted et al. BMC Res Notes (2017) 10:29, DOI 10.1186/s13104-016-2358-y QUANTIFICATION OF TWO ISOMERIC FLAVONES IN RAT COLON TISSUE USING REVERSE PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY Crystal L. Whitted1, Victoria E. Palau1,
Fecha de publicación 07 de enero de 2017
Fecha de aceptación 19 de diciembre de 2016
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BMC Research Notes

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