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THE EFFECTS OF FURANOXAPALAMINE ON COLON CANCER CELLS AS A NOVEL TREATMENT OPTION


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Científica

Tipología

Investigación y estudios

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Eventos: Congreso

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APPALACHIAN STUDENT 2017 RESEARCH FORUM April 11 - 12, 2017 D. P. Culp Center at ETSU • Johnson City, TN


THE EFFECTS OF FURANOXAPALAMINE ON COLON CANCER CELLS AS A NOVEL TREATMENT OPTION


Caden W. Cox1, Anna Lee1, Danielle Eskens1, Ruben D. Torrenegra2, Oscar Rodriguez3, Austin C. Gardner1, and Victoria Palau1.


1 Department of Pharmaceutical Sciences, Gatton College of Pharmacy, East Tennessee State University, Johnson City, TN; 2 Department of Environmental Science and Technology, Universidad de Ciencias Aplicadas y Ambientales, Bogota, Colombia; 3 Department of Environmental Engineering, El Bosque University, Bogota, Colombia.




Colon cancer is the 3rd most common type of cancer in the United States with an estimated 134,000 new cases and 49,000 deaths in 2016. Recent studies have found efficacy in compounds isolated from indigenous plants in eliciting cytotoxic effects on cancerous cells. The primary objective of this study was the isolation and examination of a furanoeremophilane isolated from the plant Senecio pampae, and its cytotoxic effects on colon cancer cell lines. The compound was isolated using a combination technique of column chromatography and thin layer chromatography. Caco2 and HCT-116 colon cancer cell lines were grown to ~70% confluency and dosed with either DMSO used as a vehicle or 5, 10, 20, 40, or 80 μM solutions of furanoxapalamine dissolved in DMSO. After 24 hours, the cells were analyzed using MTT assay and spectrophotometry to test for mitochondrial function and cell viability. In order to confirm our data and determine whether furanoxapalamine caused apoptosis or necrosis, the cells were also analyzed using annexin V and propidium iodide via fluorescence microscopy. Furanoxapalamine was then compared to 5-FU, the most common treatment for colon cancer. The results show a significant decrease in cell viability in Caco2 and HCT-116 colon cancer cells treated with furanoxapalamine compared to control. In addition, preliminary results suggest the compound shows more effective cytotoxicity than the current standard of 5- FU. This is shown by an EC50, using furanoxapalamine in Caco2 and HCT-116 colon cancer cell lines, of 10.0 μM and 48.7 μM respectively. When treated with the standard treatment of 5-FU, the EC50 values were 86.6 μM and 144.4 μM respectively for Caco2 and HCT-116 colon cancer cell lines, which is in agreement with data published in previous studies. This research indicates the potential viability and need for further study of furanoxapalamine as chemotherapeutic treatment for colorectal cancer.

Autores

Caden W. Cox1, Anna Lee1, Danielle Eskens1, Ruben D. Torrenegra2, Oscar Rodriguez3, Austin C. Gardner1, and Victoria Palau1.

Registro ISMN

ETSU-019-16 .25M

SNIES Área

Pharmacology, Toxicology and Pharmaceutics

SNIES Categoría

Cancer Research

Fecha de publicación 11 de abril de 2017
Fecha de aceptación 12 de marzo de 2017
Medio indexado (nombre)

Bases de datos donde está referenciada

http://www.etsu.edu/studentresearch/documents/Call_for_Abstracts.pdf

English information
Title

THE EFFECTS OF FURANOXAPALAMINE ON COLON CANCER CELLS AS A NOVEL TREATMENT OPTION

Abstract

APPALACHIAN STUDENT 2017 RESEARCH FORUM April 11 - 12, 2017 D. P. Culp Center at ETSU • Johnson City, TN

THE EFFECTS OF FURANOXAPALAMINE ON COLON CANCER CELLS AS A NOVEL TREATMENT OPTION

Caden W. Cox1, Anna Lee1, Danielle Eskens1, Ruben D. Torrenegra2, Oscar Rodriguez3, Austin C. Gardner1, and Victoria Palau1.

1 Department of Pharmaceutical Sciences, Gatton College of Pharmacy, East Tennessee State University, Johnson City, TN; 2 Department of Environmental Science and Technology, Universidad de Ciencias Aplicadas y Ambientales, Bogota, Colombia; 3 Department of Environmental Engineering, El Bosque University, Bogota, Colombia.

Colon cancer is the 3rd most common type of cancer in the United States with an estimated 134,000 new cases and 49,000 deaths in 2016. Recent studies have found efficacy in compounds isolated from indigenous plants in eliciting cytotoxic effects on cancerous cells. The primary objective of this study was the isolation and examination of a furanoeremophilane isolated from the plant Senecio pampae, and its cytotoxic effects on colon cancer cell lines. The compound was isolated using a combination technique of column chromatography and thin layer chromatography. Caco2 and HCT-116 colon cancer cell lines were grown to ~70% confluency and dosed with either DMSO used as a vehicle or 5, 10, 20, 40, or 80 μM solutions of furanoxapalamine dissolved in DMSO. After 24 hours, the cells were analyzed using MTT assay and spectrophotometry to test for mitochondrial function and cell viability. In order to confirm our data and determine whether furanoxapalamine caused apoptosis or necrosis, the cells were also analyzed using annexin V and propidium iodide via fluorescence microscopy. Furanoxapalamine was then compared to 5-FU, the most common treatment for colon cancer. The results show a significant decrease in cell viability in Caco2 and HCT-116 colon cancer cells treated with furanoxapalamine compared to control. In addition, preliminary results suggest the compound shows more effective cytotoxicity than the current standard of 5- FU. This is shown by an EC50, using furanoxapalamine in Caco2 and HCT-116 colon cancer cell lines, of 10.0 μM and 48.7 μM respectively. When treated with the standard treatment of 5-FU, the EC50 values were 86.6 μM and 144.4 μM respectively for Caco2 and HCT-116 colon cancer cell lines, which is in agreement with data published in previous studies. This research indicates the potential viability and need for further study of furanoxapalamine as chemotherapeutic treatment for colorectal cancer.

Keywords

colon cancer

Información de apoyo a la difusión
Documentos 2017_ASRF_ProgramBook_resumen.pdf
Etiquetado como: COLON CANCER

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