Seroprevalence of Toxoplasma gondii in Pregnants Patients from a Large Public Hospital in Bogota, Colombia

Científica

Investigación y estudios

Digital: Memorias

Memorias VIII Congreso Colombiano de Enfermedades Infecciosas

To determine the prevalence of IgG and IgM antibodies against Toxoplasma gondii in pregnant women in district 18 of Bogotá, Colombia.

Methods: Patient data was collected from Hospital Rafael Uribe Uribe, a large public hospital in the south of Bogotá, Colombia, from January 2005 to December 2006. IgG and IgM values were determined at the hospital using commercially available enzyme-linked immunosorbent kits (Diagnostic Systems Laboratories,Inc.).

Results and conclusions: Six thousand one hundred and three patients were surveyed during the period of study; 2,531 (41%) patients were IgG seropositive. In the case of 950 patients (16% of the total number of patients), IgM measurements were requested by the attending physician, and of this selected group of patients, 44 (4.6% of IgM tested) were IgM seropositive. We isolated DNA from 24 of the IgM seropositive blood samples. In one of these samples we were able to detect parasite DNA by amplification of the T. gondii B1 gene (Grigg and Boothroyd, 2001). Five of the IgM positive samples were submitted to IgG avidity measurements and exhibited high avidity. We gratefully acknowledge the generous cooperation of Hospital Rafael Uribe Uribe which made possible this study.

HORTÚA-TRIANA M.A.
CÁRDENAS S.
JUEZ G.
GARAVITO M.
ZIMMERMANN B.H.

0123-9392

Biochemistry, Genetics and Molecular Biology

Cell Biology

Seroprevalence of Toxoplasma gondii in Pregnants Patients from a Large Public Hospital in Bogota, Colombia

To determine the Prevalence of IgG and IgM antibodies against Toxoplasma gondii in pregnant women in district 18 of Bogota, Colombia.
Methods: Patient data was collected from Hospital Rafael Uribe Uribe, a large public hospital in the south of Bogotá, Colombia, from January 2005 to December 2006. IgG and IgM values ​​were determined to at the hospital using Commercially available enzyme-linked immunosorbent assay kits (Diagnostic Systems Laboratories, Inc.).
Results and conclusions: Six thousand one hundred and three patients were Surveyed During the period of study, 2,531 (41%) were IgG seropositive patients. In the case of 950 patients (16% of the overall number of patients), IgM measurements were requested by the attending physician, and of this selected group of patients, 44 (4.6% of IgM tested) were IgM seropositive. We isolated DNA from 24 of the IgM seropositive blood samples. In one of These samples We were able to detect parasite DNA by amplification of the T. gondii B1 gene (Grigg and Boothroyd, 2001). Five of the IgM positive samples were submitted to IgG avidity measurements and exhibited high avidity. We gratefully acknowledge the generous cooperation of Rafael Uribe Uribe Hospital Which made ​​this study possible.

Toxoplasma gondii, prevalence, pregnant muejeres, anti-Toxoplasma IgG antibodies